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. 2016 Aug 22;6:31777. doi: 10.1038/srep31777

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PHH were infected with JFH1 (MOI of 2.5) for 6 h and then treated with 50 μM quercetin (JFH1 + Q50 μM), or DMSO as carrier control, for 24 h (a) or 72 h (b,c). (a) Cells were lysed for quantification of negative-strand HCV RNA. (b,c) Culture supernatant were collected for determination of extracellular HCV RNA level and infectivity titer, respectively. Results are expressed as percentage of carrier control. Data are presented as the mean values ± SD obtained from two to three independent experiments.