Figure 4. Effect of quercetin on DGAT gene expression level and activity.

Huh-7.5 cells were infected with JFH1 (1 MOI) for 72 h in presence or not of 50 μM quercetin. (a) DGAT mRNA expression levels were determined by RT-PCR. Results were normalized using GAPDH and DMSO-treated non-infected cells were used as reference. *p < 0.05; **p < 0.01 and ***p < 0.001 (b) DGAT activity (ratio to DMSO-treated cells, standardized to non-infected cells). *p < 0.05 and **p < 0.01. Data are the mean value ± SD obtained from three independent experiments.