Table 1. . Ultra-performance LC parameters for the relative quantitation of globotriaosylceramide isoforms/analogs in mice tissues.
| Parameters | Description |
|---|---|
| Column |
Acquity UPLC HSS T3 |
| Dimensions |
2.1 mm × 50 mm (1.8 µm) |
| Column temperature |
30°C |
| Injection mode |
Partial loop with needle overfill |
| Injection volume |
7.5 µl |
| Autosampler temperature |
10°C |
| Mobile phase A |
MeOH + 5 mM Amm. Form. + 0.1% FA |
| Mobile phase B |
H2O + 5% MeOH + 5 mM Amm. Form. + 0.1% FA |
| Weak wash |
50:50 phase A:phase B |
| Strong wash | MeOH + 0.2% FA |