FIG 4 .
YfiN interacts with FtsZ and ZipA in E. coli. (A) Localization of EYfiNGFP in wild-type (MG1655) cells and temperature-sensitive (ts) mutants with mutations of cell division proteins in E. coli. For each strain, cells producing EYfiNGFP were exposed to 2.5 µg/ml PMB for 30 min. Following the exposure, cells were incubated for another 30 min at two different temperatures, 30°C and 42°C, before imaging. (B) Bacterial adenylate cyclase two-hybrid (BACTH) analysis of E. coli division proteins against EYfiN. Either the T18 or T25 fragment was fused to the C-terminal ends of EYfiN and the cell division proteins. PC, positive control (T18-leucine zipper/T25-leucine zipper); NC, negative control (T18/T25 empty vectors).