TABLE 2 .

Inhibition of adenylate kinase by Ap5A to determine nucleotide specificity of acetyl-CoA synthetase activity in cell extracts of S. aciditrophicus^b

Activity	Substrate	Ap5A (mM)	Sp acta
Adenylate kinase	ADP	0	3.38 ± 0.16
		0.75	0.63
		1.5	0.02
ADP-forming acetyl-CoA synthetase	Acetyl-CoA, KPi, ADP	1.5	0.02 ± 0.003
AMP-forming acetyl-CoA synthetase	AMP, PPi, plus above	1.5	1.3 ± 0.2

^aMean ± standard deviation from triplicate determinations in units per milligram of protein.

^bAbbreviations: P_i, phosphate; PP_i, pyrophosphate.