Fig. 2.

GSK3β expression/activity modulates ethanol’s effect on cell viability. a SK-N-MC cells stably over-expressing wide type (WT), S9A mutant (S9A), and kinase-deficient (KD) GSK3β were exposed to ethanol (0 or 400 mg/dl) for 48 h. Cell viability was determined by MTT assay and expressed as percentage of non-ethanol-treated controls. * denotes significant difference from matched, non-ethanol controls. # denotes significant difference from ethanol-treated vector cells. & denotes significant difference from ethanol-treated WT cells. b SK-N-MC cells stably over-expressing WT, S9A, and KD GSK3β were pretreated with GSK3β inhibitors (lithium, 20 mM and TDZD-8, 10 µM) for 30 min, then exposed to ethanol (0 or 400 mg/dl) for 48 h. Cell viability was determined as described above. * denotes significant difference from cells that were not treated with GSK3β inhibitors. From [134]