Table 4.
Efficiency of qPCR
| Reference Genes
| |||||
|---|---|---|---|---|---|
| Assay | Threshold (dRn) | RSq (dRn) | Slope (dRn) | Efficiency (%) | Tm (°) |
| GAPDH | 0.2 | 0.992 | −3.552 | 91.2 | 83.6 |
| PPIA | 0.2 | 0.997 | −3.353 | 98.7 | 84.4 |
| CYC1 | 0.2 | 0.988 | −3.455 | 94.7 | 86.8 |
|
| |||||
| Target Genes | |||||
|
| |||||
| Assay | Threshold (dRn) | RSq (dRn) | Slope (dRn) | Efficiency (%) | Tm (°) |
|
| |||||
| PAK1 | 0.2 | 0.988 | −3.566 | 90.7 | 83.8 |
| PAK2 | 0.2 | 0.991 | −3.351 | 98.8 | 81.8 |
| PAK3 | 0.2 | 0.997 | −3.103 | 110 | 82.8 |
| RAC1 | 0.2 | 0.995 | −3.178 | 106.4 | 83.2 |
| RAC1b | 0.2 | 0.967 | −3.147 | 107.9 | 80.4 |
| ARHGEF7 | 0.2 | 0.995 | −3.333 | 99.5 | 83.25 |
| CDC42 | 0.2 | 0.99 | −3.426 | 95.8 | 79.8 |
Efficiency data for reference genes and target genes. CT values obtained from 10-fold serial dilutions of cDNA were plotted against dilution factors and the reaction efficiency was calculated using qBasePLUS software.
RSq, r-squared; Rn, normalized reporter signal; dRn, delta Rn (Rn minus the baseline); Tm, melting temperature