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Annealing sites of clonotype-specific oligonucleotide primers and probes. The nucleotide and deduced amino acid sequences of TCR β (T cell clones, P2–10, 1C2, CSF3) or α chain (T cell clone A6) CDR3 are shown. The annealing sites of oligonucleotide primers and probes for TaqMan quantification of clonotypic TCR transcripts from each T cell clone are indicated by boxes (single line, forward primer; dashed line, internal probe; double line, reverse primer).
