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Specificity of primers/probe sets
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To test the specificity of each clonotypic primer/probe combination (columns) we amplified cDNA (rows) from target T cell clones (P2–10, 1C2, A6, CSF3; 5 × 103 cells/reaction) and from controls that included additional non-target MBP-specific T cell clones (3A6, MS18, GP34, 5F6, GDBP, 5G7, 5A1) and allogeneic PBMC (PB1-PB6). Input cDNA was equivalent to 5 × 103 cells/reaction for target clones and 105 cells/reaction for non-target controls.
