Table 1.
Expression of different Gα subunits in various types of leukocytes1
| Cell type | Gnas | Gnai2 | Gnai3 | Gna12 | Gna13 | Gnaq | Gna11 | Gna15 |
|---|---|---|---|---|---|---|---|---|
| B | 12.1 | 13.7 | 2.3 | 0.3 | 3.0 | 0.4 | 0.4 | 0.1 |
| Dendritic | 8.2 | 11.8 | 2.3 | 0.5 | 2.4 | 1.4 | 0.3 | 0.1 |
| Neutrophil | 5.8 | 50.0 | 8.6 | 0.4 | 6.0 | 3.5 | 0.5 | 0.1 |
| Macrophage | 6.4 | 6.9 | 5.7 | 0.3 | 2.2 | 3.8 | 0.5 | 0.3 |
| NK | 16.1 | 13.7 | 3.3 | 0.3 | 2.0 | 0.2 | 0.5 | 0.2 |
| NKT | 6.1 | 3.5 | 2.5 | 0.1 | 3.2 | 0.2 | 0.4 | 0.1 |
| CD4 | 7.7 | 11.8 | 2.7 | 0.2 | 2.8 | 0.1 | 0.4 | 0.1 |
| CD8 | 8.9 | 7.2 | 2.7 | 0.2 | 3.0 | 0.1 | 0.5 | 0.1 |
| Tγδ | 11.0 | 6.9 | 3.6 | 0.3 | 4.1 | 0.2 | 0.3 | 0.1 |
| Treg | 6.0 | 7.1 | 3.2 | 0.3 | 3.3 | 0.3 | 0.4 | 0.1 |
Relative mRNA expression in different cell types. Cells were isolated from 3 male C57BL/6J mouse spleens with the exception of the macrophages, which were isolated from the peritoneal cavity of similar mice. Data are from the Immunological genome project (http://www.immgen.org/databrowser/index.html) and based on RNA sequencing (Illumina) of mRNA from FACS purified cell samples. Very low or undetectable expression of Gnat1, Gnat2, Gnat1, Gnai1, Gnao1, Gnaz, Gnal, and Gna14. Cell type with highest expression of the different Gα subunits is highlighted. Levels of mRNA expression for Gβ and Gγ subunits can be found at the same website. For comparison β-actin expression is approximately 400. Values for each gene (x 103).