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. 2016 Aug 22;2:16061. doi: 10.1038/cddiscovery.2016.61

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Rapamycin decreases whereas bafilomycin promotes cell death in the presence of fat. (a–c) Immunofluorescence of same cells treated with (a) no drugs, (b) rapamycin, and (c) bafilomycin. (d) Cleaved caspase-3 protein levels for cells treated with (1.0 mM) or without (0.0 mM) oleate/palmitate fatty acids with no drug, rapamycin, or bafilomycin. DAPI stains for nuclei while FITC stains for cleaved caspase-3 proteins. All immunofluorescence images were taken at ×20 magnification. Immunoblotting data are expressed in fold change of protein levels versus BSA control at 0.0 mM oleate/palmitate (*P<0.05).