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. Author manuscript; available in PMC: 2016 Aug 22.
Published in final edited form as: J Vet Intern Med. 2011 Jul-Aug;25(4):890–896. doi: 10.1111/j.1939-1676.2011.0756.x

Figure 2.

Figure 2

Characterization of LPCs by PARR. (A) Flow cytometry analysis was used to determine the efficiency of Progenitor+ cell enrichment and depletion in a B-cell lymphoma sample after immunomagnetic separation. (B) PCR analysis of Progenitor+ and Progenitor− samples using primers for Cμ, IgH, and TCRγ primers. PCR product size was analyzed by capillary electrophoresis. The number next to the peak represents the fluorescence intensity. (C) Serial dilutions of Progenitor+ and Progenitor− cell DNA were used for PARR analysis. Data show the ratio of scanned pixel intensity from the IgH product (band) over the Cμ product. For DNA from LPC-enriched cells, the ratios of Cμ/IgH were 1.1, 1.1, and 1.6. For DNA from LPC-depleted cells, the values were 1.5, 1.4, and 1.0 (1 ng to 0.01 ng DNA input samples, respectively). Similar results were seen in two dogs.