| Volume of Stock Solution | Final Concentration |
| 5 mL of antibiotic-antimycin (100×) | 100 U/mL penicillin 100 μg/mL streptomycin 0.25 μg/mL amphotericin |
| 50 μL of EGF (0.1 mg/mL) | 10 ng/mL EGF |
| 50 mL of inactivated FBS | 10% FBS |
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