Volume of Stock Solution	Final Concentration
5 mL of antibiotic-antimycin (100×)	100 U/mL penicillin 100 μg/mL streptomycin 0.25 μg/mL amphotericin
50 μL of EGF (0.1 mg/mL)	10 ng/mL EGF
1 mL of 2.5 mg/mL insulin,	5 μg/mL insulin
2.5 mg/mL transferrin, and	5 μg/mL transferrin
2.5 μg/mL sodium selenite	5 ng/mL sodium selenite
25 μL of FGF (10 μg/mL)	5 ng/mL FGF