Skip to main content
. Author manuscript; available in PMC: 2016 Nov 25.
Published in final edited form as: Sci Transl Med. 2016 May 25;8(340):340ra74. doi: 10.1126/scitranslmed.aac4296

Fig. 5. miR-31 targets dystrophin and nNOS mRNA.

Fig. 5

(A) Effect of an miR-3' TSB on the dystrophin 3′UTR (n = 9) [versus non-targeting control (NC); n = 7] on dystrophin and nNOS transcripts in Ago2 immunoprecipitates in atrial myocytes from patients with AF. Data are medians and interquartile ranges. P values were determined by Wilcoxon matched pairs test. (B) Effect of an miR-31 TSB for site 5 on the nNOS 3'UTR (n = 9) versus NC (n = 7) on dystrophin and nNOS transcripts in Ago2 immunoprecipitates in atrial myocytes from patients with AF. Transcripts were normalized to miR-31. Data are averages ± SEM. P values were determined by paired t test. (C) Effect of an miR-31 mimic (n=4 to 7) or NC (n = 4 per group) on the rate of decay of nNOS and dystrophin mRNA (normalized to GAPDH) in actinomycin D–treated atrial myocytes from patients in SR (expressed as percentage of the respective mRNA at 0 hour). Data are averages ± SD. ***P < 0.001 versus NC at the respective time point; P value is also given for the interaction between treatments (NC and miR-31 mimic) and time, by two-way ANOVA with Bonferroni correction. (D and E) Effect of nNOS[site5]-TSB (n = 8) or NC (n = 5) on nNOS or dystrophin mRNA (D) and protein content (E) in atrial myocytes from patients with AF. Data are averages ± SEM except for nNOS in (D), which are medians and interquartile ranges. P values were determined by unpaired t test or Mann-Whitney U test [for nNOS in (D)]. (F and G) Effect of dystrophin-TSB (n = 5) or NC (n = 5) on nNOS or dystrophin mRNA (F) and protein content (G) in atrial myocytes from patients with AF. Data are averages ± SEM except for nNOS in (G), which are medians and interquartile ranges. P values were determined by unpaired t test or Mann-Whitney U test [for nNOS in (G)].