Figure 3.

Effect of divalent cations on percentage of fusion between liposomes and intact Pseudomonas aeruginosa ATCC 25619 correlated to atomic radius.

Notes: Two milliliters of bacteria grown to an OD_{660 nm} of 0.6 in Mueller Hinton broth was then mixed with the labeled liposomes and 1 mM of each ion to a final concentration of 10Å µg/mL. Fusion was monitored by Rh fluorescence decrease at 590 nm after 30 minutes resulting from resonance energy transfer efficiency decrease. Data are shown as mean ± standard deviation (n=3). *Significance achieved for P<0.05. ATCC^® 25619™ (American Type Culture Collection, Rockville, MD, USA).

Abbreviations: Rh, rhodamine; OD, optical density.