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. 2016 Aug 22;12(8):e1005844. doi: 10.1371/journal.ppat.1005844

Fig 1. EBV modulates expression of E2F6 and other E2F family members.

Fig 1

A) 10 million human peripheral blood mononuclear cells (PBMC) were infected with BAC-GFP-EBV for 4 hours. Cells were harvested at indicated time periods, and total RNA was isolated and reverse transcribed to cDNA according to the manufacturer’s instructions. Quantitative Real-time PCR was performed to detect E2Fs transcription levels. B) Bar graph shows log2 fold changes of E2F1 and E2F6 mRNA expression in detail that were analyzed previously. C-D) 10 million HEK-293 or Saos-2 cells were co-transfected with Myc-EBNA3C and control plasmid or Flag-tagged E2F1, E2F2, E2F3a, E2F4, E2F6 expression plasmids. Transfected cells were harvested and lysed at 36 hours post-transfection. About 5% of total lysates were reserved as the input. After incubating with 1 μg of mouse anti-Flag (M2) antibody, Immunoprecipitations were prepared for detection. Input and immunoprecipitated proteins were identified with 10% SDS-PAGE and analyzed by western blot using specific antibodies.