Fig 10. E2F6 is critical for EBNA3C-mediated cell proliferation.

A-B) 1 million BJAB cells were infected with the indicated lentivirus in combination 20 μg/ml polybrene. After 72 hours incubation, puromycin antibiotic was added as a 0.5 μg/ml concentration for selection. The selected cells were observed the GFP immunofluorescence (A) and the target protein expressions were detected by western blot (B). C-D) 1 million BJAB7 cells were infected and selected for 2 weeks using 0.5 μg/ml puromycin. GFP immunofluorescence (C) was checked and the target proteins were also analyzed by western blot (D). E) 3 million BJAB or BJAB7 stable cell lines were treated with 5μM CFSE solution for 10 minutes at room temperature. Then cells were washed, cultured, and harvested at indicated time points (0, 24, 48, and 72h). Flow cytometry was used to analyze CFSE-labeled cells.