Table 1. Antibodies used in this work.
| Primary antibody (Source) | Dilution (v/v)1 | Secondary antibody | Dilution (v/v) | Application2 |
|---|---|---|---|---|
| Rabbit anti-MATα1 (Mingorance et al. [29]) | 1:10000 | Goat anti-rabbit IgG (BioRad; 170–6515) | 1:10000 | WB |
| Rabbit anti-MATα1 (Mingorance et al. [29]) | 1:1000 | Goat anti-rabbit Alexa Fluor 546 (Molecular Probes; A11035) | 1:400 | IF |
| Rabbit anti-MATβ (Abcam; ab109484) | 1:2000 | Goat anti-rabbit IgG (BioRad; 170–6515) | 1:10000 | WB |
| Mouse anti-FLAG (Sigma; F3165) | 5 μg/ml | Anti-mouse IgG (GE Healthcare; NA931) | 1:20000 | WB |
| Mouse anti-FLAG (Sigma; F3165) | 5 μg/ml | Goat anti-mouse Alexa Fluor 488 (Molecular Probes; A11029) | 1:400 | IF |
| Mouse anti-TBP (Santa Cruz; sc-56796) | 1:1000 | Anti-mouse IgG (GE Healthcare; NA931) | 1:20000 | WB |
| mouse anti-tubulin (Sigma; T9026) | 1:2500 | Anti-mouse IgG (GE Healthcare; NA931) | 1:20000 | WB |
| Mouse anti-HA (Covance; MMS-101R) | 1:1000 | Anti-mouse IgG (GE Healthcare; NA931) | 1:20000 | WB |
| Mouse anti-HA (Covance; MMS-101R) | 1:1000 | Goat anti-mouse Alexa Fluor 488 (Molecular Probes; A11029) | 1:400 | IF |
| Mouse anti-GST (Cell Signaling; #2624) | 1:10000 | Anti-mouse IgG (GE Healthcare; NA931) | 1:20000 | WB |
| Mouse anti-SC35 (BD PharMingen; 556363) | 1:1000 | Goat anti-mouse Alexa Fluor 546 (Molecular Probes; A11030) | 1:400 | IF |
| Chicken anti-MATα2 (Abcam; ab26174) | 1:10000 | Goat anti-chicken IgY (Abcam; ab6877) | 1:2000 | WB |
| Rat anti-HA (Roche; 11-867-423-001) | 100 ng/ml | Goat anti-rat Alexa Fluor 488 (Molecular Probes; A11006) | 1:500 | IF |
| Rabbit anti-PDRG1 (Abcam; ab121219) | 1:1000 | Goat anti-rabbit IgG (BioRad; 170–6515) | 1:1000 | WB3 |
1Dilution expressed as (v/v), except where otherwise indicated
2WB, western blotting; IF, immunofluorescence.
3Exposed on ultrasensitive film.