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. 2016 Aug 22;11(8):e0161396. doi: 10.1371/journal.pone.0161396

Fig 4. Migration and invasion of RMS cells is influenced by furin activity.

Fig 4

A) Impact of furin activity on wound healing. Rh30 and RD cells with stable modulation of furin activity were used in a scratch assay. Pictures were taken at 0h, 4h, 16h and 24h and wound healing was assessed over time 0h using the program TScratch. Depicted are mean values ± SD. B) Effect of furin activity on RMS cell invasiveness. Rh30 and RD cells (wt, fur, pdx, shFA and shFE) were cultured on fluorescently labeled gelatin-coated 8-chamber slides for 24h and 48h, respectively. Treatment with 50 μM of the pan-PC inhibitor decanoyl-RVKR-chloromethylketone (CMK) was started 16h prior plating of the cells. Immunofluorescent pictures were taken and percentage of invasion was calculated based on degradation of fluorescent gelatin using the program ImageJ. Depicted are mean values ± SD. Student t-test, p<0.05 *, p<0.01 **, p<0.005 ***.