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. 2016 Aug 22;11(8):e0161396. doi: 10.1371/journal.pone.0161396

Fig 5. Maturation of furin substrates and IGF1R signaling are furin activity dependent in RMS cells.

Fig 5

A) Maturation of furin substrates upon treatment with pan-PC inhibitor CMK. Rh30 and RD cells were treated with 50 μM CMK for 16h and maturation status of the furin substrates IGF1Rβ, VEGF-C, PDGF-BB and MT1-MMP were examined by immunoblotting. B) IGF1Rβ maturation in Rh30 and RD cell lines. Levels of precursor and mature IGF1Rβ were analyzed in stable Rh30 and RD cell lines (wt, fur, pdx, shFA and shFE) by immunoblotting. C) IGF signaling in RD cells upon transient furin silencing. RD cells were transiently transfected with control (scr) or anti-furin siRNA (siFur1 and siFur2) and serum starvation was started 24h post transfection. Cells were stimulated for 10 min with 50 ng/mL IGF1 48h post transfection and maturation status of IGF1Rβ and phosphorylation of Akt (Ser473) were assessed by immunoblotting.