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Rabbit muscle triose phosphate isomerase (1 U/mL) was added and an equilibrium ratio of 18:1 for DHAP:GAP was used to assign a concentration of GAP.
| Substrate tested (for determination of Km) | Concentration of substrates used in each reaction | ||
|---|---|---|---|
| ThDP (mM) | DHAP (μM) | Pyruvate (mM) | |
| ThDP | 0‒1 | 263 | 5 |
| GAP | 0.1 | 0–197 | 5 |
| Pyruvate | 0.1 | 263 | 0–1 |
