Fig 3. Principal Component analysis of the 41 metagenomes using the top eleven variables identified from the Random Forest analysis.

Turf taxonomic and functional potential compared with other abundant benthic organisms and seawater from Abrolhos environments. A) The major orders and B) subsystems (SEED Level 1) found in the metagenomes of the turf samples from Abrolhos reefs compared with coral and rhodolith metagenomic data. Principal Component Analysis using 11 Level 1 SEED Subsystems selected based on the “Mean Decreasing Accuracy” of the Random Forest analysis. Metagenomic relative abundance data in percentages were transformed to arcsin(√x). Previously published seawater, coral, and rhodolith metagenome datasets were used in this analysis (Bruce et al., 2012; Garcia et al., 2013; Cavalcanti et al., 2013, 2014, respectively).