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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.5.1–5B.5.16. doi: 10.1002/cpsc.14

Figure 1. Self-Cloning CRISPR Gene Editing.

Figure 1

(A) Schematic of the scCRISPR process within target cells. The self-cleaving sgPal plasmid recombines with the short PCR-amplified sgRNA template to form a new site-specific sgRNA plasmid, to facilitate genome editing.

(B) Histogram of flow cytometric GFP fluorescence (x-axis) of Hist1h3a mouse ESCs after electroporation with sgPal plasmid alone (left), or together with sgGFP homology fragment (right).

(C) Flow cytometric plot of efficient generation of Pou5f1-GFP knock-in mouse ESCs (y-axis) using PCR-amplified GFP fragment.