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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.5.1–5B.5.16. doi: 10.1002/cpsc.14

60bp scCRISPR oligo design

For 20 bp sgRNA TGGAAAGGACGAAACACCGN19GTTTAAGAGCTATGCTGGAAAC

For 21 bp sgRNA GGAAAGGACGAAACACCGN20GTTTAAGAGCTATGCTGGAAAC

For 19 bp sgRNA TGGAAAGGACGAAACACCGN18GTTTAAGAGCTATGCTGGAAACA