|
| ||
| Volume | Reagent | % Reaction Volume |
|
| ||
| 100ul | NEBNext 2× Master Mix | 50% |
|
| ||
| 10ul | Unpurified product of PCR 1 | 5% |
|
| ||
| 5ul | 20uM GFPstep2 Fw primer | 2.5% |
|
| ||
| 5ul | 20uM GFPstep2 Rv primer | 2.5% |
|
| ||
| 6ul | DMSO | 3% |
|
| ||
| 74ul | mQ water | 37% |
|
| ||
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