| ||
Volume | Reagent | % Reaction Volume |
| ||
10ul | OneTaq 2× Master Mix with standard buffer | 50% |
| ||
x.x ul | 200ng Bulk gDNA | |
| ||
0.5ul | 20uM Validation primer Fw | 2.5% |
| ||
0.5ul | 20uM Validation primer Rv | 2.5% |
| ||
x.x ul | mQ water | Up to 100% |
|
| ||
Volume | Reagent | % Reaction Volume |
| ||
10ul | OneTaq 2× Master Mix with standard buffer | 50% |
| ||
x.x ul | 200ng Bulk gDNA | |
| ||
0.5ul | 20uM Validation primer Fw | 2.5% |
| ||
0.5ul | 20uM Validation primer Rv | 2.5% |
| ||
x.x ul | mQ water | Up to 100% |
|