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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.5.1–5B.5.16. doi: 10.1002/cpsc.14

Volume Reagent % Reaction Volume

10ul OneTaq 2× Master Mix with standard buffer 50%

x.x ul 200ng Bulk gDNA

0.5ul 20uM Validation primer Fw 2.5%

0.5ul 20uM Validation primer Rv 2.5%

x.x ul mQ water Up to 100%