|
| ||
| PCR step | Primer | Sequence |
|
| ||
| Step 1 | sgRNA_HDRstep1_fw | TGTTTTAAAATGGACTATCATA TGCTTACCGTAACTTGAAAGTATTTCGATTTCT TGGCTTTATATATCTTGTGGAAAGGACGAAACACC |
|
| ||
| Step 1 | sgRNA_HDRstep1_rv | GTTGATAACGGACTAG CCTTATTTAAACTTGCTATGCTGTTTCCAGCATAGCTC TTAAAC |
|
| ||
| Step 1 | sgRNA_HDRstep2_fw | GTACAAAATACGTGACGTAGAAA GTAATAATTTCTTGGGTAGTTTGCAGTTTT AAAATTATGTTTTAAAATGGACTATCATATGCTTACC |
|
| ||
| Step 1 | sgRNA_HDRstep2_rv | ATTTTAACTTGCTATTTCTAGCTCT AAAACAAAAAAGCACCGACTCGGTGCCAC TTTTTCAAGTTGATAACGGACTAGCCTTATTTAAAC |
|
| ||
| Step 2 | sgRNA_HDRstep3_fw | CGATACAAGGCTGTTAGAGAGATA ATTAGAATTAATTTGACTGTAAACACAAA GATATTAGTACAAAATACGTGACGTAGAAAGTAATAA |
|
| ||
| Step 2 | sgRNA_HDRstep3_rv | TCAATGTATCTTATCATGTCTGC TCGATTTTAACTTGCTATTTCTAGCTCTAAAA CAAAA |
|
| ||
| Step 3 | sgRNA_HDRstep4_fw | GGCCTATTTCCCATGATTCCTT CATATTTGCATATACGATACAAGGCTGTTAGA GAGATA |
|
| ||
| Step 3 | sgRNA_HDRstep4_rv | TCAATGTATCTTATCATGTCTGCTCGA |
|
| ||
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