|
| ||
| Volume | Reagent | % Reaction Volume |
|
| ||
| 10ul | OneTaq 2× Master Mix with standard buffer | 50% |
|
| ||
| 0.5ul | 20uM stock of 60bp sgRNA oligo | 2.5% |
|
| ||
| 1ul | 10uM HDRstep1 Fw + Rv primer mix | 5% |
|
| ||
| 1ul | 10uM HDRstep2 Fw + Rv primer mix | 5% |
|
| ||
| 7.5ul | mQ water | 37.5% |
|
| ||
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