|
| ||
| Volume | Reagent | % Reaction Volume |
|
| ||
| 10ul | OneTaq 2× Master Mix with standard buffer | 50% |
|
| ||
| 1ul | Unpurified product of PCR 1 | 5% |
|
| ||
| 1ul | 10uM HDRstep3 Fw + Rv primer mix | 5% |
|
| ||
| 8ul | mQ water | 40% |
|
| ||
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