|
| ||
| Design | Primer | Sequence |
|
| ||
| (Pre-STOP 40bp) – GFP_Fw | GFPstep1_Fw |
TGTTCCCGTCACTGCTCTGGGCTCTCCCATGCATTCAAAC GTGAGCAAGGGCGAGGAGCT |
|
| ||
| (Post-STOP 40bp) – GFP_Rv | GFPstep1_Rv |
CCAGGTTCTCTTGTCTACCTCCCTTGCCTTGGCTCACAGC TGAGGAGTGAATTGCGGCCG |
|
| ||
| (35–40bp ext) – GFP_step1_Fw | GFPstep2_Fw |
TCTACTCAGTCCCTTTTCCTGAGGGCGAGGCCTTTCCCTC TGTTCCCGTCACTGCTCTGG |
|
| ||
| (35–40bp ext) – GFP_step1_Rv | GFPstep2_Rv |
TAATCCCTCCTCAGTAAAAGAATTTAACCCCAAAGCT CCAGGTTCTCTTGTCTACCTCCC |
|
| ||
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