|
| ||
| Volume | Reagent | % Reaction Volume |
|
| ||
| 10ul | NEBNext 2× Master Mix | 50% |
|
| ||
| 0.1ul | 100ng/ul stock of GFP template | 0.5% |
|
| ||
| 0.5ul | 20uM GFPstep1 Fw primer | 2.5% |
|
| ||
| 0.5ul | 20uM GFPstep1 Rv primer | 2.5% |
|
| ||
| 0.6ul | DMSO | 3% |
|
| ||
| 8.3ul | mQ water | 41.5% |
|
| ||
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