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. 2016 Jul 19;15(16):2145–2156. doi: 10.1080/15384101.2016.1195531

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Dynein 1 heavy chain 1 (DHC1) and dynein 1 light intermediate chain 2 (LIC2) interacts with BRCA2 in centrosomes. (A) Centrosomes were isolated from HeLa S3 cells by sucrose density gradient centrifugation. The centrosome-containing fractions were analyzed by immunoblotting using the anti-γ-tubulin antibody. BRCA2 was detected in centrosome-containing fraction 7. (B) Anti-BRCA2 and anti-IgG immunoprecipitates were subjected to in-solution digestion and analyzed by liquid chromatography-tandem mass spectrometry. Mass spectra for the DHC1 peptide (AA 1440–1452), LIC2 peptide (AA 143–159), and BRCA2 peptide (AA 2606–2615) in anti-BRCA2 immunoprecipitates.