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. 2016 Jul 19;15(16):2145–2156. doi: 10.1080/15384101.2016.1195531

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — The effects of HA-CLS-DsRed on the cell cycle. (A) The cell-cycle phases (right panels) of HA-CLS-DsRed- and HA-DsRed-positive HeLa S3 cells (boxed areas: Left panels) were measured by BrdU incorporation into DNA using flow cytometry. (B) Western blot analysis showing cyclin A and Cdk2 expression in HA-CLS-DsRed- and HA-DsRed-transfected cells. Cell extracts were used for anti-cyclin A immunoprecipitations. The immunoprecipitates were immunoblotted and probed with anti-Cdk2 antibody to detect Cdk2 expression in Cdk2-cyclin A complexes from HA-CLS-DsRed- and HA-DsRed-transfected cells. Presented results are representative of 3 experiments. The histograms show quantification of Cdk2 expression. (C) Kinase assays of Cyclin A immunoprecipitations from cells transfected with HA-CLS-DsRed and HA-DsRed. The Cdk2-cyclin A activity was measured by Kinase-Glo Plus Luminescent Kinase Assay (Promega). Presented results are representative of 3 experiments. The histograms present quantified luminescence, which is related to kinase activity.