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H3 T118ph localization to the centrosome is dependent on PLK-1, and occurs in worms and flies. (A) Drosophila embryos at the syncytial blastoderm stage in mitosis. White box indicates magnified area. Arrows mark centrosomes. (B) Control RNAi and PLK-1 (RNAi)-depleted C. elegans 4-cell embryos. Scale bars = 5 μm. White box indicates magnified prometaphase cell. (C) Immunofluorescence analysis of H3 T118ph in untreated and BI-2536 treated prometaphase HeLa cells. Scale bar = 5 μm.
