Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1985 Sep;38(9):1045–1051. doi: 10.1136/jcp.38.9.1045

IgG enzyme linked immunosorbent assay for diagnosis of invasive aspergillosis: retrospective study over 15 years of transplant recipients.

A K Trull, J Parker, R E Warren
PMCID: PMC499356  PMID: 3900143

Abstract

Four commercially available Aspergillus fumigatus antigens were compared in an IgG enzyme linked immunosorbent assay by testing multiple sera from 19 patients who had received renal and hepatic allografts and who had histologically confirmed invasive aspergillosis. Raised antibody levels were found in 16 of the 19 patients. More antibody responses were detected by culture filtrate antigens, but somatic antigens often detected antibodies sooner. When antibodies to Aspergillus antigens were present before transplantation the onset of fatal disseminated infection was earlier. Antibody levels often fell to within the normal range, even in cases of fulminant disseminated infection. Repeated serology was essential for detection of an antibody response.

Full text

PDF
1048

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coleman R. M., Kaufman L. Use of the immunodiffusion test in the serodiagnosis of aspergillosis. Appl Microbiol. 1972 Feb;23(2):301–308. doi: 10.1128/am.23.2.301-308.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gold J. W., Fisher B., Yu B., Chein N., Armstrong D. Diagnosis of invasive aspergillosis by passive hemagglutination assay of antibody. J Infect Dis. 1980 Jul;142(1):87–94. doi: 10.1093/infdis/142.1.87. [DOI] [PubMed] [Google Scholar]
  3. Gurwith M. J., Stinson E. B., Remington J. S. Aspergillus infection complicating cardiac transplantation. Report of five cases. Arch Intern Med. 1971 Oct;128(4):541–545. [PubMed] [Google Scholar]
  4. Holmberg K., Berdischewsky M., Young L. S. Serologic immunodiagnosis of of invasive aspergillosis. J Infect Dis. 1980 May;141(5):656–664. doi: 10.1093/infdis/141.5.656. [DOI] [PubMed] [Google Scholar]
  5. Marier R., Smith W., Jansen M., Andriole V. T. A solid-phase radioimmunoassay for the measurement of antibody to Aspergillus in invasive aspergillosis. J Infect Dis. 1979 Nov;140(5):771–779. doi: 10.1093/infdis/140.5.771. [DOI] [PubMed] [Google Scholar]
  6. Mishra S. K., Falkenberg S., Masihi K. N. Efficacy of enzyme-linked immunosorbent assay in serodiagnosis of aspergillosis. J Clin Microbiol. 1983 Apr;17(4):708–710. doi: 10.1128/jcm.17.4.708-710.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rinaldi M. G. Invasive aspergillosis. Rev Infect Dis. 1983 Nov-Dec;5(6):1061–1077. doi: 10.1093/clinids/5.6.1061. [DOI] [PubMed] [Google Scholar]
  8. Schaefer J. C., Yu B., Armstrong D. An aspergillus immunodiffusion test in the early diagnosis of aspergillosis in adult leukemia patients. Am Rev Respir Dis. 1976 Mar;113(3):325–329. doi: 10.1164/arrd.1976.113.3.325. [DOI] [PubMed] [Google Scholar]
  9. Young R. C., Bennett J. E. Invasive aspergillosis. Absence of detectable antibody response. Am Rev Respir Dis. 1971 Nov;104(5):710–716. doi: 10.1164/arrd.1971.104.5.710. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES