Figure 1.

Schematic of ORF2 and tagged ORF2 fragments. The ORF2p molecule has multiple annotated domains important for retrotransposition. These include the enzymatically necessary endonuclease (EN: light blue) and reverse transcriptase (RT: purple) domains. Between the EN and RT domains is the Cryptic region (Cry: dark blue) and Z domain (Z: orange). Both contain amino acids essential to retrotransposition and ORF2p function. At the C-terminal end of the ORF2p is a cysteine-rich domain (Cys: yellow) that also contains amino acids essential to retrotransposition. EN-containing ORF2 fragments detailed here were generated in 2 formats: one with an N-terminal Gal4 tag and one with an N-terminal VP16 tag. VP16-tagged expression plasmids also contain a neomycin resistance gene. EN239Δ, EN269Δ, EN274Δ, EN289Δ, EN347Δ, and ENZ490Δ were also generated in an untagged format with a Hygromycin resistance gene in the expression plasmid. As described in materials and methods, reported domains are used as the body of the name, followed by the number corresponding to the terminal amino acid as it would be in the full length ORF2p, with the truncated ORF2p sequence denoted by a Δ.