Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

letter

. 2016 Jun 20;6(4):e1198300. doi: 10.1080/2159256X.2016.1198300

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Author(s). Published with license by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 2. — Chronic and Acute Toxicity of EN-containing ORF2 fragments. VP16-tagged and Gal4-tagged EN-containing ORF2 fragments are toxic to HeLa cells. (A) Chronic and Acute toxicity assays of EN-containing ORF2 fragments in HeLa cells. Amino acid that ORF2 fragment terminates in (ENΔ) denoted below graph bars for both functional (black) and nonfunctional (gray) EN-containing ORF2 fragments. Nonfunctional EN-containing fragments have catalytically necessary amino acids mutated in the EN domain (D205A, H230A). Error bars denote standard deviation (n = 3). Statistical significance was assessed using Student's t-test (*p < 0.05). (B) Grouping of chronic toxicity. Colony counts for EN264Δ and EN269Δ are not significantly different from one another. Colony counts for EN274Δ and EN279Δ are not significantly different from one another. Colony counts for EN264Δ and EN269Δ are significantly different from colony counts for EN274Δ and EN279Δ. Statistical significance was assessed using Student's t-test (*p < 0.05). (C) Grouping of acute toxicity. Colony counts for EN239Δ, EN264Δ and EN269Δ are not significantly different from one another. Colony counts for EN274Δ, EN279Δ, EN289Δ, EN314Δ, EN330Δ, EN347Δ, and ENZ490Δ are not significantly different from one another. Colony counts for EN239Δ, EN264Δ and EN269Δ significantly different from colony counts for EN274Δ, EN279Δ, EN289Δ, EN314Δ, EN330Δ, EN347Δ, and ENZ490Δ. Statistical significance was assessed using Student's t-test (*p < 0.05).