Figure 3.

Subcellular distribution of Gal4-tagged EN-containing ORF2 fragments. Western blot analysis of EN-containing ORF2 fragments. Indicated Gal4-tagged EN-containing constructs were transiently transfected into HeLa cells and subjected to Western blot analysis. Both whole cell lysate and cytoplasmic and nuclear cell fractions were analyzed using commercially available anti-Gal4 antibodies, yielding a single band in each sample lane corresponding to the expected size of each ORF2p fragment. Control is cells transfected with empty vector. Cell fractions are indicated on the right. Molecular weight markers are indicated on the left. GAPDH (total cell lysate and cytoplasmic fraction) and Lamin A/C (L A/C: nuclear fraction) were used as loading controls.