Table 2. Standardized SDM using mutagenic primers of 29-mer length and different GC content and Tm.
| PTEN | K147R* | K6R* | K164R* | K263R** | K144R* | K313R* | K183R | K62R* | K60R* | K322R | K66R | ||
| % GC | 52 | 48 | 45 | 41 | 41 | 38 | 38 | 34 | 31 | 28 | 24 | ||
| Tm | 76 | 74 | 73 | 71 | 71 | 70 | 70 | 68.5 | 67 | 66 | 64.5 | ||
| Colonies | exp 1 | 59 | 73 | 80 | 7 | 70 | 86 | 63 | 111 | 87 | 25 | 71 | |
| Mutation frequency | exp 1 | 3/3 | 3/3 | 2/3 | 2/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 1/3 | 3/3 | |
| PTPRZ-B | C1328R | E1213K | V848A** | I1133M | L1291V** | S1453A* | G917D | A1220D* | Y922H | F1246L | V1389M* | P1281L | |
| % GC | 52 | 48 | 41 | 41 | 41 | 41 | 38 | 38 | 34 | 34 | 34 | 24 | |
| Tm | 76 | 74 | 71 | 71 | 71 | 71 | 70 | 70 | 68.5 | 68.5 | 68.5 | 64.5 | |
| Colonies | exp 2 | 34 | 36 | 108 | 8 | 77 | 99 | 66 | 74 | 90 | 124 | 170 | 135 |
| Mutation frequency | exp 2 | 3/3 | 2/3 | 1/3 | 0/3 | 2/3 | 3/3 | 2/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
All mutations have one nucleotide mismatch with respect to the wild type sequence. Data are represented as in Table 1. pRK5-PTEN or pENTR-PTPRZ-B served as template plasmids.
*One of the primers from the mutagenic primer pair has G or C in 3’ position.
**Both primers from the mutagenic primer pair have G or C in 3’ position.