Figure 1. Effects of TFIIAγ5 on the expression of disease susceptibility genes Os8N3, Os11N3, TFIIAγ1, or OsTFX1, after Xoo infection.
Plants were inoculated with Xoo strain PXO99 (harbouring TALEs PthXo1, PthXo7, and PthXo6), PXO86 (harbouring TALE PthXo3) or PXO61 (harbouring TALE AvrXa7) at the booting (panicle development) stage. It is known that PthXo1, PthXo7, and PthXo6 induce Os8N3, TFIIAγ1, and OsTFX1, respectively, and PthXo3 and AvrXa7 all induce Os11N3. Each bar represents mean (three replicates) ± standard deviation. (A) Mutation of TFIIAγ5 (rice line IRBB5). b, significant difference between IR24 and IRBB5 at p<0.01. (B) TFIIAγ5-RNAi lines. b, significant difference between wild-type (WT) and transgenic plants at p<0.01.
Figure 1—figure supplement 1. Effects of TFIIAγ5 on rice resistance to Xoo strains known to carry TALEs.
Rice plants at the booting (panicle development) stage were inoculated with Xoo. (A) The near-isogenic lines IR24 and IRBB5 showed different responses to the infection of Xoo. IRBB5 in IR24 background carries a natural mutated TFIIAγ5, TFIIAγ5V39E. Each bar represents mean (total 17 to 29 leaves from 5 plants) ± standard deviation. b, significant difference between IR24 and IRBB5 at p<0.01. (B) The enhanced resistance of TFIIAγ5-RNAi plants to strain PXO99 was associated with reduced transcription of TFIIAγ5 but not TFIIAγ1. WT, wild-type Zhonghua 11. Each bar represents mean (3 replicates for gene expression and total 5 to 10 leaves from one plant for lesion length) ± standard deviation. b, significant difference between wild-type (WT) and transgenic plants at p<0.01. (C) The enhanced resistance of TFIIAγ5-RNAi plants co-segregated with reduced TFIIAγ5 transcription in T1 families. Each bar represents mean (3 replicates for gene expression and 5 to 10 leaves from one plant for lesion length) ± standard deviation. b, significant difference between WT and transgenic plants at p<0.01. (D) TFIIAγ5-RNAi plants showed enhanced resistance to all the Xoo strains. Each bar represents mean (total 35 to 40 leaves from 5 plants) ± standard deviation. b, significant difference between WT and transgenic plants at p<0.01.
Figure 1—figure supplement 2. Effect of TFIIAγ5 on Xa23-mediated resistance to Xoo strain PXO99.
Each bar represents mean (total 40 to 45 leaves from 5 plants for lesion length and 3 replicates for gene expression) ± standard deviation. (A) Xa23 Xa23- mediated resistance required the presence of TFIIAγ5/TFIIAγ5 or TFIIAγ5/TFIIAγ5V39E. Rice plants at the booting stage were inoculated with Xoo. b, significant difference between IR24 and other plants at p<0.01. (B) PXO99 infection-induced Xa23 expression required the presence of TFIIAγ5/TFIIA5 or TFIIAγ5/TFIIA5V39E. b, significant difference between non-inoculated plants (ck) and PXO99-inoculated plants within each rice line at p<0.01. 1d and 2d, 1 day or 2 days after inoculation of PXO99.