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. 2016 Jul 29;5:e19605. doi: 10.7554/eLife.19605

Figure 3. Effects of the TFB region of TALE PthXo1 on the virulence of Xoo strains and on the expression of rice susceptibility gene in rice−Xoo interaction.

Each bar represents mean (total 30 to 35 leaves from five plants for lesion length; three replicates for gene expression and bacterial growth rate) ± standard deviation. (A) Virulence of wild-type strain T7174 and recombinant strains carrying PthXo1 and its derivatives in IR24 and IRBB5. b, significant difference between T7174 and recombinant strains in each rice line at p<0.01. (B) Growth of different Xoo strains in rice leaves. b, significant difference between 0 day (30 min after infection) and 12 days after infection of each strain at p<0.01. (C) Expression of susceptibility gene Os8N3 after infection of different strains. b, significant difference between non-inoculated (ck) and inoculated (at 48 hr after infection of a strain) plants in each rice line at p<0.01.

DOI: http://dx.doi.org/10.7554/eLife.19605.010

Figure 3—source data 1. Effects of leucine residues of PthXo1 TFB region on TALE-mediated infection.
DOI: 10.7554/eLife.19605.011

Figure 3.

Figure 3—figure supplement 1. Effects of the TFB region of TALE PthXo1 on the virulence of Xoo strains and on the expression of rice susceptibility gene in rice−Xoo interaction. .

Figure 3—figure supplement 1.

Each bar represents mean (total 30 to 35 leaves from five plants for lesion length; three replicates for gene expression and bacterial growth rate) ± standard deviation. (A) Virulence of wild-type strain KACC10331 and recombinant strains carrying PthXo1 and its derivatives in near-isogenic lines IR24 and IRBB5. b, significant difference between T7174 and recombinant strains in each rice line at p<0.01. (B) Growth of different strains in rice leaves. b, significant difference between 0 day (30 minutes after infection) and 12 days after infection of each strain at p<0.01. (C) Expression of susceptibility gene Os8N3 after infection of different strains. b, significant difference between non-inoculated (ck) and inoculated (at 48 hr after infection of a strain) plants in each rice line at p<0.01.