Each bar represents mean (total 30 to 35 leaves from five plants for lesion length; three replicates for gene expression and bacterial growth rate) ± standard deviation. (A) Virulence of strain PH and its derivatives carrying pthXol or modified pthXo1 in near-isogenic lines IR24 and IRBB5. PH is an engineered TALE-free strain with the genetic background of strain PXO99A, which carries pthXo1. b, significant difference between PH and its derivatives in each rice line at P < 0.01. (B) Growth of different strains in rice leaves. b, significant difference between 0 day (30 minutes after infection) and 12 days after infection of each strain at P < 0.01. (C) Expression of susceptibility gene Os8N3/SWEET11 after infection of different strains. b, significant difference between non-inoculated (ck) and inoculated (at 48 h after infection of a strain) plants in each rice line at P < 0.01.
DOI:
http://dx.doi.org/10.7554/eLife.19605.028