Figure 7. mAChR stimulation of glutamatergic neurons modulates peptide release from varicosities at the ring gland upon protein-deprivation.
(A) Time series of ANF::GFP release from varicosities in the ring gland of the indicated genotypes at the indicated time intervals, after stimulation by Carbachol (CCh). (B–D) Traces represent a time series of mean normalized peptide ( ± SEM) release from varicosities at the ring glands of the indicated genotypes after Carbachol (CCh) stimulation. (E) Box plots representing CCh-stimulated peptide release with ANF::GFP quantified by area under the curve of the indicated genotypes on PDD from (B–D). Box plots and symbols are as described for Figure 3F. F and G Traces represent a time series of mean normalized peptide release (ANF::GFP; ± SEM) from varicosities at the ring glands of indicated genotypes upon Carbachol (CCh) stimulation. (H) Box plots representing CCh-stimulated peptide release with ANF::GFP quantified by area under the curve of the indicated genotypes on PDD from (F and G.). Box plots and symbols are as described for Figure 3F. Bars with the same alphabet represent statistically indistinguishable groups (one-way ANOVA with a post hoc Tukey’s test p<0.05).