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. Author manuscript; available in PMC: 2017 May 19.
Published in final edited form as: Mol Cell. 2016 May 19;62(4):491–506. doi: 10.1016/j.molcel.2016.04.020

Figure 7. SEC16A mutant with the phosphomimetic S846D overcomes the defect in ER-to-Golgi trafficking and prevents activation of the UPR in ULK-deficient cells.

Figure 7

(A) Mean percentages (±SEM) of cells with nuclear staining of CHOP. Data were acquired from 3 independent experiments, and more than 100 GFP+ cells per population were scored in each experiment. *P <0.001 (ANOVA) when compared with empty vector-transduced cells. (B) Representative pseudocolored images of WT and Ulk1-ko MEFs transfected with indicated GFP-tagged cargo [i.e, (−), empty vector; VSVG-GFP or SERT-GFP] stained with an antibody against CHOP and DAPI. Scale bar: 10 μm. (C) Mean percentages (±SEM) of cells with nuclear staining of CHOP. Data were acquired from 3 independent experiments, and more than 100 GFP+DDK+ cells per population were scored in each experiment. *P <0.001 (ANOVA) when compared with empty vector- transduced cells. SA: S846A SEC16A mutant; SD, S846D SEC16A mutant. (D) Representative pseudocolored images of MEFs stained with antibodies against MYC-DDK-tagged SEC16A (channel not shown) and CHOP and DAPI. Scale bar: 10 μm. See also Figure S7.