Figure 5. Overexpression of DISC1 suppresses synaptic development in larval NMJ.
A. Branching patterns of wild-type and DISC1 overexpression (DISC1OE) NMJs. Green, motor neuron termini labeled with an anti-neuronal antibody (anti-HRP). Magenta, synaptic boutons labeled with anti-Synaptotagmin (SYT). Muscle 6/7 of the second abdominal segment in late third instar larvae (116–120 hours after egg collection). UAS-DISC1 was driven with a ubiquitous driver, tubP-GAL4. Scale bar, 40 μm.
B–D. Quantification of NMJ synaptogenesis in DISC1 overexpression NMJ. B. Total synaptic bouton area. C. Number of synaptic boutons. D. Number of axonal branching points. *p < 0.05 and **p < 0.01 with one-way ANOVA and Dunnett’s post hoc test, n = 10 ~26.