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Effect of Pde4 inhibition on hepatic Cpt1a expression and cAMP/pCREB levels. (A) Immunohistochemical staining with anti-CPT-1A antibody (×20 final magnification). (B) Cpt1a mRNA levels after 4 wk of feeding. (C) Hepatic cAMP levels after 2 wk of alcohol feeding. (D) Nuclear pCREB staining of livers after 4 wk of alcohol feeding. Data are presented as the mean ± SD (n=5–7 mice per group). *P<0.05, ** P< 0.01, *** P< 0.001.
