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Extracellular (A), membrane (B), and periplasmic (C) protein fractions of R. leguminosarum bv. trifolii Rt24.2, Rt2472, and Rt2472(pRC24) separated by 1D electrophoresis. Proteins (10 μg) were loaded on each lane. Prestained protein ladder, 10–250 kDa (Thermo Scientific), was used as a molecular weight marker.
