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. 2016 Aug 23;7:1302. doi: 10.3389/fmicb.2016.01302

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Copyright © 2016 Rachwał, Boguszewska, Kopcińska, Karaś, Tchórzewski and Janczarek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blotting of extracellular, membrane, periplasmic, and cytoplasmic protein fractions of R. leguminosarum bv. trifolii Rt24.2 and Rt2472 strains (A). The assay was performed using polyclonal rabbit antibodies against 32-kDa cytoplasmic inositol monophosphatase PssB and 43-kDa outer membrane lipoprotein PssN with N-terminal domain directed to the periplasmic space. Ten micrograms of individual protein fractions was loaded on each lane. Abbreviations: 24- Rt24.2, 2472- Rt2472. (B) Two-dimensional electrophoretic profiles of extracellular proteins of Rt24.2 and Rt2472. Hundred micrograms of individual protein fractions were analyzed (molecular weight marker—prestained protein ladder, 10–250 kDa, Thermo Scientific). Spots containing proteins in higher amounts which were excised from the gel are marked by red arrows, whereas those containing diminished protein amounts were marked by blue circles.