FIGURE 8.

Effect of PrCa and GA₃ applications on arbuscular distribution according to the different morphological classes, arbuscule abundance in the whole root system (%A) and SlPT4 gene expression in roots of sitiens tomato plants colonized with R. irregularis. After 1 week of transplanting and inoculation with R. irregularis, a set of sitiens tomato plants (control) were treated with 0.1 % ethanol solution, and three sets of plants were treated with ABA, GA₃, and GA₃+ABA. GA₃ (5 μM) and ABA (75 μM) solutions were applied to soil twice per week, and plants were harvested 50 days after inoculation. qPCR data represents the expression of target gene in treated plants with respect to the expression in non-treated control plants in which expression was designated as 1. Values correspond to mean ± SE (n = 3). Bars with same letters do not significantly differ (P = 0.05) according to Duncan’s multiple range test.